Evolution of neoplasic cells

NOTE: This is a research paper which was printed in Germany and was sent to us translated


                           "Entwicklung neoplasticher Zellkulturen unter dem Einfluss elektromagnetishcer Felder"

Journal : Erfahrungs Heilkunde, Acta Medica Empirica
July 1997 pp. 398-404
Publisher : Haug (Karl F. Haug Verlag, Germany)

Karl F. Haug Verlag (Verlag means Publisher)
email : webadmin@huethig.de
The Journal "Erfahrungs Heilkunde" is at :


             Evolution of neoplasic cells in culture under the influence of electromagnetic fields


Jean-Claude Mainguy
Medical doctor, ex-director of Moanda Hospital (Gabon)

Sylvie Crochet

Jean-Marie Danze
Chemical science degree, ex-teacher at Liege University (Institute of
Pharmacy), Biophysics consultant


The simultaneous application of pulsed low frequency magnetic fields and of specific electromagnetic signals emitted from preparations of malignant tissues lysates and from plants extracts, both diluted according to a particular method, induces the almost complete destruction of malignant cells in vitro.


Since around 1925, several researchers, some of them Nobel laureates, estimated that the mechanisms of life are governed by electromagnetic fields. These fields may have an endogenous or exogenous origin. This would suggest that living systems function like transmitting/receiving radio devices. The endogenous fields are seen by these researchers as a « cellular
language » capable of transmitting from organ to organ commands of functions and specific biochemical reactions. One precursor was E. Schrödinger. He states in his book « What is life ? » (1) a series of postulates that help to understand the relations between physics and biology.

H. Fröhlich, professor at Liverpool University, well known for his studies of supraconductivity, proposed models that integrate effects of very low energy electromagnetic radiation in functional biological processes (2). He showed as physically plausible the hypothesis that weak but coherent electromagnetic fields may initiate and sustain vital processes in living organs. This concept, based on the low energy required in order to get resonance mechanisms, appears, according to Fröhlich and his disciple C.W. Smith (professor at Salford University, GB) (3,4) as one of the new paradigms to be adopted in future years in order to understand life’s secrets. 1991 Nobel laureates B. Sakmann and E. Neher demonstrated the selective behavior of cellular membranes, and confirmed the notion of «cells dialogue ».

I. Prigogine expanded our notions of biological mechanisms when he showed indirectly, through « dissipative structures », that these mechanisms obey the rules of open systems in physics. Prigogine’s views (5), similar to those of F.A. Popp (6,7,8), enable us to see a direct relation between cellular or organic functions and information coming from the environment. This information may be carried by electromagnetic frequencies included in the whole spectrum, from very low frequencies (E.L.F.) to radio frequencies, hyper frequencies, infrared and light frequencies. Any living being may now be considered as a system that may respond directly to external stimuli transmitted as electromagnetic radiation at low energy, for instance.

So life might be simultaneously structured, organized (negative entropy) and destructured, disorganized (positive entropy) by electromagnetic fields at low energy, depending on the type of radiation received by the organism or some of his cellular tissues.

Experiments performed by Adey (9,10) in the USA on healthy blood cells showed that proteic strings emerging from cell walls may tap weak electric fields and transmit them to the inside of cells. W.R. Adey and his collaborators also observed that radio frequencies influence cells when modulated by frequencies under 100 Hertz.

They showed that signals drowned in the ambient background electromagnetic noise are selectively perceived by cells when these frequencies match a particular system of cell resonance. This lead to think that the electromagnetic resonance model, similar to the one used in radio communications (coherent systems), corresponds well to cellular systems.
Adey’s team also demonstrated that the magnetic component of the fields is the most biologically active.

In the same line of thinking we may quote a paper by P.A. Anninos, N.Tsagas, R. Sandyk and K. Derpapas, at Demokritas Reseach Center in Athens, Greece. They show that magnetic fields may constitute a treatment for partial loss of consciousness (11). Frequency range is 2 to 7 Hertz.

The orthopedic surgery department at Columbia University, New York, under the initiative of R.O. Becker and C.A.L. Basett, has been using for many years pulsed magnetic fields for consolidating fractures suffering from pseudoarthrose (chronic non-consolidation). More than 250 000 patients have been successfully treated with this non-invasive method. Results were
controlled with double-blind studies.

In a recent publication, C.A. Basett notes that preliminary data suggest possible rapid advances for the control of malignant tumors (12) with low intensity pulsed magnetic fields.

These considerations led us to confront the problem in a slightly different way and attempt to influence tumor cell cultures using specific electromagnetic fields.


The goal of this study is the exploration of the following concepts :

- Is it possible to destroy in vitro cancerous cells of very diverse strains, by exposing them to pulsed magnetic fields, to which    one superimposes specific electromagnetic radiation ?

- If so, in which proportion the cancerous cells are destroyed ?

- What becomes of healthy cells during this experiment ?

- Is there an electromagnetic « cellular language », whose nature is common to all animal cells ? Does each type of cell have a    specific « language » ? (3,4,13)

The present research aims to explore this specific language and use it on tumor cell cultures, in order to influence their metabolism and selectively destroy them.

Equipment and methods

We always treat simultaneously healthy cells and tumor cells belonging to the same organ, in order to evaluate the effect of the treatment on each kind of cell.

Five organs have been selected for this study : lung, breast, liver, colon, kidney.

Tumor cell strains are :
- lung : small cell cancer DMS 53
- breast : chemioresistant mammary cancer ZR 75-1
- liver : adenocarninoma S.K. Hep 1
- colon : adenocarcinoma LOVO (Multi-Drugs Resistant) M.D.R.
- kidney : carcinoma A. 498

All strains are from human origin and come from ATCC Bethesda, M.D., USA.

The place where the experiment is done has fundamental importance.

In the light of preliminary tests, it appeared that the 50 Hertz electric and magnetic fields generated by electrical apparatus and by electrical wiring have a negative influence on the experimentation’s reproducibility. We measured ambient fields (14) and selected in the laboratory an electromagnetically silent place.

Our criteria for background noise (extremely low frequency : 50 Hertz) :
- electric field : less than 0,7 Volt per meter (resolution : 0,1 V/m)
- magnetic field : 0 mG (sensitivity limitation of the measuring device : 0,1 mG)

Measures of ambient electric and magnetic fields (50 Hz) were made with a calibrated device EFM 130 (Electric Fields Measurements W. Stockbridge, MA 01266, USA).

All cells are cultured on 6-pits plates (Falcon), 48 hours before actual experimentation, in order to obtain confluent cells in single layer.

Cells are cultured without any antibiotic.

Each strain is cultured simultaneously 4 times in identical pits, in order to obtain a greater uniformity of cultures and better reliability in results’ interpretation. 2 pits are used for counting and 2 for observation.

Cell counting is done by a Counter Coulter apparatus.

Microscope is made by Leitz.

Magnifications are :
- weak magnification : x 10
- strong magnification : x 20
- global magnification : x 10 x 0.32

Treatment applies simultaneously
1 / pulsed magnetic fields
2 / specific radiation

Magnetic fields have the following characteristics :
- pulsed fields whose impulsion trains follow a fixed cadence. These pulses have peak width of 1 microsecond and are  unipolar.
- induction field produced at a distance of 10 cm of the inductive device is between 0,4 and 0,6 milligauss (0,04 to 0,06 microtesla)

Specific radiation is picked up from diluted preparations of tumoral tissues extracts and vegetal extracts lysates, through a system of metal electrodes and container. It is amplified by a large-band amplifier with minimal distortion of frequencies et very slow phase shifting while functioning. The amplifier is capable or amplifying all frequencies while maintaining constant peak width. (A MORA device satisfying these criteria was used in mode A).

The amplifier runs on a battery, in order to avoid perturbing fields generated by the 50 Hz grid. Cables between input and output electrodes are screened.

As soon as the device is on, the amplifier picks up and amplifies signals coming from the input, thus including specific frequencies of tumor lysate dilutions and vegetal extracts dilutions placed in the electrode. These frequencies are transferred to the output with alternately 3 seconds amplification and 6 seconds pause.

Frequency band is 1 Hz to 150 kHz.

Signals transmitted to the cell culture are :
- those of the device itself (its own radiation and electromagnetic background noise)
- those of frequencies picked up and amplified through the amplifier device, coming from diluted preparations of tumor tissue lysates (belonging to the same strain as the target cells), to which we add combinations of vegetal extracts.

It is obvious that the signals emitted by the materials in the input electrodes are drowned in the background noise of the amplifier and ambient fields. It is impossible to distinguish them but, following W.R. Adey’s work quoted in the introduction, we think it is rational to postulate that cell cultures are able to distinguish them, through resonance. Our experiment will enable us to confirm it, on the basis of actual effects.

Duration of treatment is 60 minutes per session.

For the study presented here, we repeated the treatment for 5 days, one session per day.

Microscope examination of target cells is done after 120 hours.

We insist on the following facts :
- Absolutely no ionizing radiation was applied
- No chemical substance was applied before or during experimentation.


Healthy cells were not altered by the various fields applied during this study.

Observed on strong magnification microscope, each cell kept its original

The only notable modification was a light stimulation of growth, about 10 %, as shown is fig. 2, left side graphic, for kidney cells, and fig. 3, left side graphic, for lung cells.

Only malignant tumor cells were destroyed. Percentage of diminution of their number after 48 hours is between 70 and 90 %. Destruction is selective.

This destruction of tumor cells reinforced itself spontaneously in the course of time, as revealed by examination after 120 hours (fig. 4)

Examination of tumor cells with strong magnification showed after 120 hours a very clear vacuolization of the cytoplasma, and a densification of the cell walls, indicating imminent death.

Study on glioblastoma

In this research, another study was done on a brain tumor with very high malignity, the glioblastoma.

Here again we used tumor cells of human origin : glioblastoma U251. As we did not possess healthy human brain tissue, we used healthy astrocytes from ferrets. Both strains, malignant and healthy, were simultaneously submitted to the same protocol as the one previously used on the 5 tumor strains (lung, breast, liver, colon, kidney).

The only difference was the introduction of specific glioblastoma information, from dilution of lysates of these tumor cells.

Results of the study on glioblastoma.

Healthy cells were not modified in a significant way during the 6 days of experimentation, in quality or quantity. 10 % variations were non significant, because of the small value of this number and because fluctuations went in both directions from day to day.

Destruction of tumor cells was between 80 % and 100 %.

During these 6 days we never saw any sign of resumption, even minimal, of neoplasic cell proliferation.


Only the simultaneous exposure of tumor cells to the specific amplified radiation and to the pulsed magnetic fields allowed us to observe the high level destruction of tumor cells. Separate use of each field produced mediocre results.

The second originality of this study lies in the use, as signal emitters, of preparations made of tumor tissue lysates et vegetal extracts. These were diluted by successive gradients, which seems to confer them a particular spectrum of electromagnetic emissions. Undiluted lysates and extracts did not manifest the destroying action.

As the amplifier, because of his conception and construction, is only able to pick up and amplify signals between 1 Hz and 150 kHz, it is obvious that this frequency band is involved in the observed results.

It is necessary to refine the study of different parameters, in order to explain the variations of percentages of destroyed cells in spite of a rigorously identical protocol.

The hour of treatment application during the day is a non negligible factor. Most of the observed phenomena on cell cultures indicate a chronobiological parameter with a nycthemeral rhythm.

We cannot either exclude the hypothesis that the electronic devices we used may have fluctuating efficacy according to the length of use (warming, shift and lowering of the peaks, etc).

In the process of diminution or complete disappearance of tumor cells, the proportion caused by direct cytotoxic effect and the proportion caused by the inhibition of cellular reproduction have not been yet evaluated.

In the hypothesis of destruction by direct cytotoxicity, each parameter should be reexamined in order to be able to regulate de process of cellular lysis, keeping in mind that too rapid a lysis may liberate a great quantity of toxins detrimental to the organism.


This study, despite its imperfection, demonstrates in an irrefutable way that it is possible to destroy almost totally in vitro cancerous human cells by appropriate electromagnetic signals.

The diversity of strains, requiring each time particular signals, indicates that the effect comes an electromagnetic « language », as evoked by H. Frohlich (2).

Healthy cells keep their perfect integrity.

It seems to us it is now legitimate to think that a new way is opening for cancer research.

We do not exclude the thought that this methodology might apply to other fields of therapeutic research.


(1) Schrödinger E. « What is life ? » ED. Cambridge University Press, 1967
(2) Fröhlich H. « Biological coherence and response to external stimuli »
Springer Verlag, Heidelberg, 1988
(3) Smith C.W. « Electromagnetic and magnetic vector ; potential
bio-information and water » in « Ultra high dilutions, pp. 187-201, Kluwer
Academic Publ., Amsterdam, 1994
(4) Smith C.W. « Electromagnetic phenomena in living biomedical systems »
Proc. 6th Ann. Conf. IEEE Eng. In Med. And Biol. Soc. Pp 176-180, 15-17 sept
(5) Prigogine I. and Stengers I. « La nouvelle alliance » (Ed Gallimard,
Paris, 1979)
(6) Popp F.A. « Biologie des Lichts » (P. Parey Verlag, Berlin, 1984)
(French trad. JM Danze, Ed. Pietteur, Liège, 1989)
(7) Popp F.A., Li K.H. and Gu Q. « Recent advances in biophoton research and
its applications (Ed World Scientific, 1992)
(8) Bischof M. « Biophotonen in unsere Zellen » (Zweitausendeins Verlag,
Frankfurt, 1995)
(9) Adey W.R. « Frequency and power windowing in tissue interactions with
weak electromagnetic fields » (Proc. IEEE, 68 (1) pp 119-125, 1980)
(10) Adey W.R., Bawin F.M., Lawrence A.F. « Effects of weak,
amplitude-modulated fields on calcium efflux from awake cat cerebral cortex
» (Bioelectromagnetics, 3, pp. 295-308, 1982).
(11) Anninos P.A., Tsagas N., Sandyk R., Derpapas K. « Magnetic stimulation
in the treatment of partial seizures » (Intern. J. Neuroscience, vol 60, pp
141-171, 1991).
(12) Basett C.A.L. « Beneficial effects of electromagnetic fields » (J. of
Cellul Biochemistry, 51, pp 387-393, 1993)
(13) Endler P.C. et al. « A zoological example of ultra high dilution
research. Energy coupling between the dilution and the organism in a model
of amphibia » (in « Ultra High Dilution », Kluwer Academ. Publ., Amsterdam,
pp. 39-48, 1994)
(14) Danze J.M., Le Ruz P., Santini R., Bousquet M., Mercier J.L. « Pourquoi
et comment mesurer les champs electriques et magnetiques 50/60 Hetz (Ed
Encore, Paris, 1994)


1. Schematic front view of the system (MORA device and magnetic device)

2. Kidney cells. Results of simultaneous treatment of healthy and malignant
cells, after 120 hours

3. Lung cells. Results of simultaneous treatment of healthy and malignant
cells, after 120 hours

Chart 1 : % of increase or decrease of cells number, treated and untreated
normal kidney (NRK - 49 F), cancerous kidney (A 498), normal lung (CCD-37Lu),
cancerous lung (DMS 53)

4. Evolution of tumor cells from different organs, treated and untreated,
after 120 hours

Chart 2 : % of increase or decrease of cancerous cells number, treated and
lung, breast, liver, colon, kidney

5. Evolution of cell cultures of human glioblastoma (U251) and healthy brain
cells (astrocytes Mpf)

Chart 3 : % of increase or decrease of cells number (U251 and Mpf)
after 48 hours, 72 hours, 96 hours, 120 hours, 144 hours

+ 8 microscope photos of cells



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